Fig. 2

qPCR assays of mixtures of Burkholderia and Paraburkholderia species bacteria. a qPCR assays with the 5 pairs of primers were validated against 1:1 mixture of B. cenocepacia LMG 16656 and B. multivorans LMG 13010 (left panel), 1:1 mixture of P. caledonica LMG 19076 and P. caribensis LMG 18531 (middle panel), and 1:1 mixture of B. cenocepacia LMG 16656 and P. caribensis LMG 18531 (right panel). The empty bars showed the theoretic values computed from qPCR data of single species from Fig. 1, while filled bars showed real detected values. b qPCR assays with the 4 pairs of primers (16S-F2/R2,; 16S-F3/R3,; dehp2-F6/R6,; dehp2-F7/R7,) were validated against different concentration combinations of B. cenocepacia LMG 16656 and P. caribensis LMG 18531. c The relationship of Delta-Ct values of Burkholderia sensu stricto-specific or Paraburkholderia-specific primer pairs using 16S rRNA () or dehp2 () with the relative proportion of Burkholderia and Paraburkholderia targets. Results shown are the means of three replicates with the error bars representing the standard deviations