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Fig. 2 | BMC Microbiology

Fig. 2

From: The study of metabolites from fermentation culture of Alternaria oxytropis

Fig. 2

Flowchart of chemical composition analysis of metabolites of Alternaria oxytropis. a The 12 L total fermentation broth were dissolved with appropriate amount of methanol (V = 1:1) overnight at 4 °C, the 23 L supernatant was filtered three times after addition of the alcohol extract. b The methanol and water in the supernatant are volatilized to obtain concentrated substances of Alternaria oxytropis fermentation broth. c The 150 g concentrate is added with water for the subsequent extraction of petroleum ether, chloroform, ethyl acetate and n-butanol. d Packing the column after mixing ten times the weight of the petroleum ether extract with petroleum ether; The petroleum ether phase extract is dissolved in methanol and mixed with the same quality of silica to form a powder, and then subjected to gradient elution with petroleum ether, petroleum ether: acetone (V:V = 7:3, 6:4). e The silica is mixed with chloroform and added to the column with 10 times the weight of the petroleum ether extract; the chloroform phase extract is dissolved in methanol and mixed with the same quality of silica to form a powder, and then subjected to gradient elution with chloroform, chloroform: acetone (V:V = 5:5, 10:13). f The method is the same as e, using chloroform: methanol: aqueous ammonia: water (V:V:V:V = 70:26:2:2) for elution. g The method is the same as e, chloroform: methanol: ammonia: water (9:1:0.1:0.1,8:1:0.1:0.1, 7:2:0.1:0.1, 70:26:2:2) for elution. h and i The purified samples were dissolved in methanol for GC-MS and LC-MS detection. The three independent experiments are presented

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