Table 3 Characteristics of the primers for the 7 loci of bacterium P. multocida
From: Biological characterization of Pasteurella multocida present in the Saiga population
â„– | Loci | Loci Function | Primer sequences | Product size (bp) | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
1 | аdk -adenylate cyclase | nucleotide biosynthesis | AAGGBACWCAAGCVCAAT | CACTTTTTKYGTMCCGTC | 531 |
2 | aroA - 3-phosphoshikimate 1-carboxyvinyl transferase | amino acid biosynthesis | TTTACCDGGYTCYAAAAG | CTTTYACVCGCCAGTTAT | 558 |
3 | pgi - phosphoglucose isomerase | energy metabolism; glycolysis | GCCWGTGYTKGTTGATGG | TTGKGCTGGCGCRATRAA | 609 |
4 | g6pd - glucose-6-phosphate 1-dehydrogenase | energy metabolism: pentose | CHGGYGAYYTMACTYATCG | TTTBGCGATBARTTTRTCRGC | 513 |
5 | gdhA - glutamate dehydrogenase | amino acid biosynthesis | YTTAGTTGARCCTGAACG | CTTGACCTTCAATYGTGC | 651 |
6 | mdh - malate dehydrogenase | energy metabolism; TCA cycle | AAGTTGCWGTWYTAGGTG | CCTAATTCAATATCYGCACG | 552 |
7 | deoD - purine nucleoside phosphorylase | nucleotide biosynthesis | GTGCATTTGCYGATGTTG | TGSYGTKGTTTGTTCGTG | 576 |