Skip to main content
Fig. 6 | BMC Microbiology

Fig. 6

From: Osteopontin in the host response to Leishmania amazonensis

Fig. 6

Histopathology and evaluation of the inflammasome transcripts on the site of parasite inoculation. a. Histological features of ear lesions in wild type C57BL/6+/+ and b. opn mutant C57BL/6−/− mice at 107 days p.i.. Microscopic lesions (ulceration, acanthosis and inflammation) were more severe in C57BL/6−/− than in C57BL/6+/+. Triangles : ulceration, stars : inflamation, arrow ↓ : acanthosis, c: cartilage, e: epidermis, d: dermis, Hematoxylin and Eosin (H&E), original magnification × 10, scale bar: 100 μm. C. Histological scores from C57BL/6 wild type (black lozenges) and C57BL/6−/− (grey lozenges) mice, post inoculated with 104L. amazonensis metacyclic promastigotes. Red bars indicate median values for each group. L = Lymphocytes, MF = Macrophages, N = Neutrophils (inflammatory). Number of mice studied: 6 KO; 4 WT. d. Ten thousand metacyclic promastigotes were inoculated in the ear dermis of C57BL/6 wild type (white columns) and opn mutant mice (columns with pattern). Data are from 3 representative mice for each strain at 107 days p.i. Relative quantity of transcripts is calculated by using the non-infected control tissues as the calibrator. Mean values and SDs are shown for each group. A Mann-Whitney test was performed to compare the fold changes between the two groups: *: P < 0.05; **P < 0.01

Back to article page
\