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Fig. 3 | BMC Microbiology

Fig. 3

From: Osteopontin in the host response to Leishmania amazonensis

Fig. 3

Distinct phenotypic traits of the opn mutant BMFs, in the presence of L. amazonensis parasites. a. Phenotypic evaluation of BMFs isolated from wild type (C57BL/6+/+) and opn mutant mice (C57BL/6−/−). While in the presence of opn, the pyroptosis-like phenotype is not observed, in its absence (C57BL/6−/−), 14.15 and 48% of the cells infected (INF) with the amastigotes are undergoing to a pyroptosis-like cell death (PYR) at 24 h and 48 h p.i. respectively (total cell N° counted at both time points: C57BL/6+/+: 416 and C57BL/6−/− 385). Statistical analyses are performed by parametric Z-test, comparison of two proportions. b. Immunostaining of BMFs from C57BL/6.spp1−/− mice infected with LV79 L. amazonensis amastigotes at a MOI: 4:1. Representative cell phenotypes of non-pyroptotic cells at 24 h p.i. (a-c) and at 48 h p.i. (i-iii) and cells undergoing to pyroptosis-like cell death are shown at 24 h p.i., (d-h) and at 48 h p.i., (iv-vi) (phase contrast image capture). (Lamp-1, LV79, Nuclei)

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