Fig. 3
From: Osteopontin in the host response to Leishmania amazonensis
Distinct phenotypic traits of the opn mutant BMFs, in the presence of L. amazonensis parasites. a. Phenotypic evaluation of BMFs isolated from wild type (C57BL/6+/+) and opn mutant mice (C57BL/6−/−). While in the presence of opn, the pyroptosis-like phenotype is not observed, in its absence (C57BL/6−/−), 14.15 and 48% of the cells infected (INF) with the amastigotes are undergoing to a pyroptosis-like cell death (PYR) at 24 h and 48 h p.i. respectively (total cell N° counted at both time points: C57BL/6+/+: 416 and C57BL/6−/− 385). Statistical analyses are performed by parametric Z-test, comparison of two proportions. b. Immunostaining of BMFs from C57BL/6.spp1−/− mice infected with LV79 L. amazonensis amastigotes at a MOI: 4:1. Representative cell phenotypes of non-pyroptotic cells at 24 h p.i. (a-c) and at 48 h p.i. (i-iii) and cells undergoing to pyroptosis-like cell death are shown at 24 h p.i., (d-h) and at 48 h p.i., (iv-vi) (phase contrast image capture). (Lamp-1, LV79, Nuclei)