Fig. 2

Bile Salt Hydrolase Activity of Lactobacillus mucosae Strains Compared to a Bile Deconjugating Lactobacillus reuteri. One unit of bile salt hydrolase activity is defined here as nmol of amino acid (taurine or glycine) cleaved from the relevant bile acid per mg of protein per minute. Taurine and glycine bile salt hydrolase activity are portrayed with red and black bars, respectively. Bars represent the means of triplicate experiments and error bars represent standard deviations. Images depict cell masses of each strain grown on MRS containing no bile acids (Control), MRS supplemented with taurodeoxycholic acids (Tauro) and glycodeoxycholic acid (Glyco). Strains which demonstrate bile salt hydrolase activity towards a particular bile acid are shown with a positive sign (+), while lack of activity is shown with a negative sign (−). Weak activity is indicated by +/−