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Fig. 7 | BMC Microbiology

Fig. 7

From: Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection

Fig. 7

In ovo synthetic resiquimod treatment increases the production of IL-1β from macrophages in lungs in vivo. SPF eggs were treated in ovo with resiquimod (n = 5) or PBS (n = 4) at ED18 and the eggs were incubated until hatch. At day 1 post-hatch, lungs were preserved in OCT, sectioned and double immunofluorescent assay was performed to identify the macrophages that produce IL-1β. The quantitative data from lung is shown along with representative immunoassayed images. Student’s t-test was performed to identify group differences and the differences were considered significant at P < 0.05

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