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Fig. 6 | BMC Microbiology

Fig. 6

From: Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection

Fig. 6

Antiviral activity of synthetic resiquimod against ILTV replication is not dependent on IL-1β production. Avian macrophages, MQ-NCSU cells, were cultured in 12-well plates (1 × 106 cells per well) for 24 h and stimulated with either resiquimod (10 μg/ml) or growth media (control) in 6 replicates. The resultant MQ-NCSU cell culture supernatants were collected at 24 h post-treatment and transferred (250 μl) to LMH cell monolayer. Before transferring MQ-NCSU cell culture supernatants, 3 wells from resiquimod and control groups in LMH cells were incubated with 1.0 μg/ml of IL-1Ra for 30 min. Twenty-four hours later, the LMH cells were infected with ILTV (300 PFU/well). The plates were stained with 1% crystal violet at 5 dpi and resulting plaques were counted. The experiment was repeated two more times with the same number of replicates with similar results and the data were pooled. ANOVA with Bonferroni’s posttest for selected comparison was performed to identify the differences between groups and the differences were considered significant at P < 0.05

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