Table 2 Extracellular and intracellular MIC endpoints for 15 antimicrobials against 3 Thailand L. intracellularis isolates. The bacteria were prepared independently and tested twice. The endpoint was obtained from the median value of 3 replicates of each passage
Antimicrobials | Minimum Inhibitory Concentration (MIC) μg/ml | |||||
|---|---|---|---|---|---|---|
CUPHE01_SW13 Passages 6–7 | CUPIA01_SW13 Passages 8–9 | CUPIA02_SW13 Passages 5–6 | ||||
Intracellular activity | Extracellular activity | Intracellular activity | Extracellular activity | Intracellular activity | Extracellular activity | |
1. Amoxicillin | 8–16 | > 128 | 8 | > 128 | 16–32 | 64 |
2. Carbadox | ≤0.125 | 8 | ≤0.125–0.25 | 0.5–1 | ≤0.125–0.25 | 0.5 |
3. Chlortetracycline | > 128–64 | > 128 | > 128 | > 128 | 32 | 32 |
4. Colistin | > 128 | > 128 | > 128 | > 128 | > 128 | > 128 |
5. Enrofloxacin | 8 | > 128 | 4–8 | 64 | 16 | 32–64 |
6. Lincomycin | > 128 | > 128 | > 128 | > 128 | > 128 | > 128 |
7.Lincomycin+Spectinomycin | 128–64 | 32 | > 128 | 8–4 | 64–128 | 2 |
8. Gentamicin | > 128 | > 128 | > 128 | 128- > 128 | > 128 | 64–128 |
9. Spectinomycin | 128 | 32 | > 128 | 8–4 | > 128–128 | 4–2 |
10. Sulfamethazine | 128 | > 128 | 4–8 | > 128 | 32–64 | > 128 |
11. Tiamulin | 1 | 8 | 1 | 4 | ≤0.125–0.25 | 0.5 |
12. Trimethoprim | 64 | > 128 | > 128–128 | > 128 | > 128 | > 128 |
13. Tylosin | 8–16 | 32 | 4 | 64 | 2 | 8 |
14. Valnemulin | ≤0.125 | 0.5–1 | ≤0.125 | 0.5–0.25 | ≤0.125 | 0.25 |
15. Zinc-Bacitracin | > 128–128 | > 128 | > 128 | > 128 | > 128 | > 128 |