MrSVP, a secreted virulence-associated protein, contributes to thermotolerance and virulence of the entomopathogenic fungus Metarhizium robertsii

Table 1 PCR primers used in this study

Gene	ID^a	5′ to 3′ sequence	Notes
Primers were designed for disruption and complementation of Mrsvp
Mrsvp	Mrsvp 5′ F	CGGAATTCCAGACTTGCCAAATCCTAC
	Mrsvp 5′ R	AACTGCAGGGTGATTACCCAGTGTTCT
	Mrsvp 3′ F	GGACTAGTCTCTTCTGTCCAGGCCAATA
	Mrsvp 3′ R	GCTCTAGAACGGCTGAGGTGATGATGTA
bar	bar F	GGAGGTCAACAATGAATGCC	PCR identification of Mrsvp
bar	bar R	CCACGTCATGCCAGTTCC	Deletion transformants
Mrsvp	CMrsvp 5′ F (c)	GGACTAGTCAGTTGGTGGCACTATTTGGT
Mrsvp	CMrsvp 3′ R (d)	GCTCTAGATTACAAAGCGAGAACCAGAGC
Primers were designed for the validation of mutants
ben	ben F	ATGGCTACCTACTCCGTCGTG	PCR identification of Mrsvp
ben	ben R	CTCGTCCATACCCTCACCA	Complementation transformants
Mrsvp	Mrsvp F (a)	GATGGCAAGGGTATGGG
Mrsvp	Mrsvp R (b)	TCAGGTTAGTAGCAGAGGGAT

^aLetters in brackets refer to primer positions in Additional file 1: Figure S1A; Restriction sites are in boldface

ISSN: 1471-2180