Fig. 3

C. abortus and psittaci can attach to bovine spermatozoa. The apical area of the sperm head seems to be the preferred location for attachment. Attachment of Chlamydia spp. (C. abortus, C. psittaci,) to the apical (acrosomal) area and other parts of bovine spermatozoa during 24 h of coincubation (MOI = 1) at 35 °C. Results are depicted as mean values of 4 replicates of 50 sperms ± SD. [The top error bar indicates the error bar of “the other parts” for the respective Chlamydia spp. (only positive SD presented); the lower error bar indicates the error bar of “the apical part” (only negative SD presented).] Approximately 60% of Chlamydiae spp. particles are attached to the apical area corresponding to the acrosomal region