Skip to main content

Table 4 The Box Behnken design with observed and predicted responses

From: Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens

Run Order

pH

Inoculum size (%)

Incubation period (days)

Observed response

Predicted response

1

9

1

6

14

17.6

2

7.5

5.5

6

28

26.8

3

9

5.5

3

0

2.0

4

7.5

5.5

6

28

26.8

5

6

5.5

9

34

34.5

6

7.5

1

9

27

25.1

7

7.5

5.5

6

28

26.8

8

9

10

6

22

22.1

9

7.5

5.5

6

28

26.8

10

6

1

6

20

22.4

11

6

10

6

25

23.9

12

7.5

10

3

0

−1.1

13

7.5

1

3

0

−4.1

14

6

5.5

3

0

3.3

15

7.5

5.5

6

27

26.8

16

7.5

10

9

26

28.1

17

9

5.5

9

30

29.3

  1. Final Equation in Terms of Actual Factors:
  2. Paromomycin activity = −47.6 –(0.36 * pH) + (2.4* inoculum size) + (19* incubation period) + (0.12* pH * inoculum size)-0.23* pH * incubation period-0.26* inoculum size2–1.1* incubation period2