Table 2 Different culture media used for optimization of paromomycin production
Media composition | A1a | A2b | A3c | A4d | A5e | A6f | A7 | A8 | A9 | A10 | A11g | A12h |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
soy bean meal (g/L) | 15 | 15 | ----i | – | – | 30 | 30 | – | – | 30 | – | – |
yeast extract(g/L) | – | 1 | 3 | 10 | – | – | – | 10 | 3 | – | – | – |
Peptone(g/L) | – | – | – | 15 | 10 | – | – | 15 | – | – | – | – |
Tryptone(g/L) | – | – | 5 | – | – | – | – | – | 5 | – | – | – |
Glucose g/l | 15 | 15 | 20 | 15 | 20 | – | 20 | – | – | – | – | – |
Glycerol (ml/L) | – | 2.5 | – | – |  | 40 | – | 40 | 40 | – | – | – |
NaCl(g/L) | 5 | 5 | – | – | 5 | – | – | – | – | – | – | – |
CaCO3(g/L) | 1 | 1 | – | – | – | 5 | 5 | – | – | 5 | – | – |
NH4Cl(g/L) | – | – | – | – | – | 4 | 4 | – | – | 4 | – | – |
K2HPO4(g/L) | – | – | 1 | – | – | – | – | – | 1 | – | – | – |
KH2PO4(g/L) | – | – | 0.1 | – | – | – | – | – | 0.1 | – | – | – |
Beef extract(g/L) | – | – | – | – | 5 | – | – | – | – | – | – | – |
TSB (g/L) | – | – | – | – | – | – | – | – | – | – | 30 | – |
Soybean casein digest broth (g/L) | – | – | – | – | – | – | – | – | – | – | – | 30 |