Fig. 5

Antioxidant activity of C. nigricolor- EA extract a) DPPH scavenging activity b) Nitric oxide scavenging potential c) Hydroxyl radical scavenging activity d) Superoxide scavenging activity. Ascorbic acid at a concentration of 50 μg mL⁻¹ was used as a positive control for all the antioxidant assays. The data are derived from atleast two independent experiments each performed in triplicates (n = 6). P value was calculated by comparing the means ± SD of the % scavening activity observed with adding and without adding the extract to the reaction mixture, using Student’s T test and the statistical significance are presented as follows in the bar graph: ***,P ≤ 0.001; **P ≤ 0.01; ns, P > 0.05