Table 1 Oligonucleotide sequence of primers used for amplification of cytokines
Cytokine | Oligonucleotides sequence (5′—› 3′) | Fragment size (bp) |
|---|---|---|
IFN –γ | F: CAG CTC TGC ATC GTT TTG GGT TCT | 466 |
R: TGC TCT TCG ACC TCG AAA CAG CAT | ||
IL-11 | F: CACATGAACTGTGTTTGCCGCCTGGT | 295 |
R: GCAGCCTTGTCAGCACACCTGGGAGCTGTAGA | ||
IL – 4 | F: GAA CAG CCT CAC AGA GCA GAA GAC | 221 |
R: TGT CGA GCC GTT TCA GGA ATC | ||
IL-12p40 | F: CCA AGA ACT TGC AGC TGA AG | 355 |
R: TGG GTC TAT TCC GTT GTG TC | ||
GAPDH | F: TGACCTCAACTACATGGTTTA | 151 |
R: GCCCCACTTGATTTTGGA |