Table 2 Plasmids used in this study
From: Characterization of HicAB toxin-antitoxin module of Sinorhizobium meliloti
Names | Genotypes | References |
|---|---|---|
pBAD24 | ori ColE1 araC, PBAD bla | [49] |
pNDM220 | Mini R1, bla, lacIq, PA1/O4/O3 | [50] |
pBBR1-MCS2 | ori pBBR1, lacZα, aphA | [40] |
pET22b(+) | ori ColE1, bla, lacI, T7p | Novagen |
pKT25 | ori P15A, aac, T25 | [37] |
pKNT25 | ori P15A, aac, T25 | [37] |
pUT18 | ori ColE1 bla, T18 | [37] |
pUT18C | ori ColE1 bla, T18 | [37] |
pKT25-Zip | ori P15A, aac, T25:: Zip | [37] |
pUT18-Zip | ori ColE1 bla, T18:: Zip | [37] |
pBBara | pBBR1-MCS2, araC, PBAD | Â |
pBB-HicA | pBBR1-MCS2, araC, PBAD -hicA | This study |
pET-HicB | pET22 hicB 6 his | This study |
pET-RHH | pET22 RHH1 6 his | This study |
pET-COG | pET22 COG 6 his | This study |
pETHicAStrepHicBHis | pET22 synthetic operon | This study |
pNDM-HicB | pNDM220 hicB | This study |
pNDM-COG | pNDM220 NH2 fragment of HicB (COG1598) | This study |
pNDM-RHH | pNDM220 COOH fragment of HicB (RHH) | This study |
pKThicA | pKT25 hicA | This study |
pKThicB | pKT25 hicB | This study |
pNThicA | pKNT25 hicA | This study |
pNThicB | pKNT25 hicB | This study |
pUThicA | pUT18 hicA | This study |
pUThicB | pUT18 hicB | This study |
pUChicA | pUT18C hicA | This study |
pUChicB | pUT18C hicB | This study |
pKTCOG | pKT25 COG1598 | This study |
pNTCOG | pKNT25 COG1598 | This study |
pKTRHH | pKT25 RHH1 | This study |
pNTRHH | pKNT25 RHH1 | This study |
pUTCOG | pUT18 COG1598 | This study |
pUCCOG | pUT18C COG1598 | This study |
pUTRHH | pUT18 RHH1 | This study |
pUCRHH | pUT18C RHH1 | This study |