Oxytetracycline reduces the diversity of tetracycline-resistance genes in the Galleria mellonella gut microbiome

Table 1 G. mellonella larvae collected from the feeding procedure

Oxytetracycline dose ⇓	1	2	3	4	5
0	+	+	+	+	+
1 mg/100 g	+	+	+	+	+
10 mg/100 g	+	+	–	–	+
100 mg/ 100 g	+	–	+	–	+
400 mg/ 100 g	+	–	+	–	+

At least 10 larvae were collected per feeding group. In some cases the larvae in an antibiotic-treated group died (−) and not only could no larvae be collected, but also no larvae reached maturity and laid eggs. In the interest of continuing the experiment, in such cases eggs laid by the adults from the control (no drug) group were moved to the food spiked with oxytetracycline. (+) indicates survival and that a sample could be collected

ISSN: 1471-2180