Material | Description | Reference or source |
---|---|---|
Bacteria | ||
 L. casei | Plasmid free | ATCC393 |
 E. coli DH5α | Transformation host | CCTCC AB 2012883 |
 L. casei/pPG6121.-BLF | Secreted BLF producer | This work |
 L. casei/pPG6121 | Transformation host | This work |
Plasmid | ||
 pUC57 | Ampr, ori | Sangon (China) |
 pPG612.1 | Ampr, Cmr, ori | Li et al. |
 pPG612.1-BLF | Ampr, Cmr, ori | This work |
Primers | ||
 BamHI-F | TGGATCTTGCTTTGAAGGGT | This work |
 BamHI-R | TTTCGTCCCATAGATCTCTGC | This work |
 XhoI-F | ACACGTGAAACAGGTGCTGCT | This work |
 XhoI-R | TTCGTCCAACCAAACCGACT | This work |