Fig. 5

Expression of MAVS suppress BEFV replication in BHK-21 cells. a Detection of MAVS protein expression by western blot assays after transfection with flag-tagged MAVS recombinant plasmid, the miR-3470b inhibitor and corresponding control groups, respectively. b Flag-tagged MAVS recombinant vector, the miR-3470b inhibitor and corresponding control groups was transfected in BHK-21 cells followed by infection with BEFV at an MOI of 0.1 for 24 h. Then, viral titers in the cell cultures were measured by TCID₅₀. Data are representative of three indcdent experiments and presented as means ± SD. ***p < 0.001, **p < 0.01, and *p < 0.05. c BHK-21 cells were transfected with siNC, siMAVS respectively, and then MAVS protein levels in siRNA-transfected cells were determined by western blotting. Numbers below the image was used for quantifications of protein blot intensities by gray value analysis derived from Image J software. d BHK-21 cells transfected with siNC, siMAVS#1 and siMAVS#2 for 24 h and then followed by infection with BEFV (0.1MOI). Subsequently, viral titers in the cell cultures were measured by TCID₅₀. Data are representative of three independent experiments and presented as means ± SD. ***p < 0.001, **p < 0.01, and *p < 0.05