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Fig. 4 | BMC Microbiology

Fig. 4

From: MiR-3470b promotes bovine ephemeral fever virus replication via directly targeting mitochondrial antiviral signaling protein (MAVS) in baby hamster Syrian kidney cells

Fig. 4

MiR-3470b downregulated the endogenous expression of MAVS. a RT-qPCR for detection of MAVS mRNA expression. The efficacy of the miR-3470b mimics or miR-3470b inhibitor and irrelevant-targeting negative control in regulating the mRNA levels of MAVS expression were detected by SYBR Green based RT-qPCR assay. b Western Blot for analysis of MAVS protein level. The cells transfected with miR-3470b mimics, miR-3470b inhibitor, irrelevant-targeting negative control and BHK-21 cells infected with BEFV (MOI of 0.1) for 36 h were harvested and subjected to analyze MAVS protein levels with rabbit anti-MAVS and β-actin antibodies for western blotting analysis. Numbers below the image was used for quantifications of protein blot intensities by gray value analysis derived from Image J software

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BMC Microbiology

ISSN: 1471-2180

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