Fig. 3

MAVS is one of the targets of miR-3470b. a Schematic of the reporter gene construction and the putative seed matches between miR-3470b and the MAVS 3’UTR. The MAVS 3’UTR contain wild or mutant types of the seed match of miR-3470b constructed and inserted into the luciferase reporter pmirGLO vector are indicated. b 50 nM of the indicated miRNA mimics or 50 nM of the indicated miRNA NC mimic was co-transfected with 50 ng of the indicated reporter constructs in 293 T cells. Firefly and Renilla luciferase activity levels were measured at 24 h post-transfection. The activity of firefly luciferase was normalized to that of Renilla luciferase. The data are representative of three independent experiments and presented as means ± SD. **p < 0.01, and *p < 0.05