Table 1 List of primers and probe for the lateral-flow strip and real-time RPA assay based on the HAdVs strain accession no. KX289874
From: Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay
Assay format | Oligo name | Sequence 5′-3’ | Genome position |
|---|---|---|---|
LFD-RPA | ADV-RF-Bio | Bio-GCTCATTGGAACAACTGCCGTAAATAGTGT | 20,761–20,790 |
ADV-RR | GGCCGAGAACGGTGTACGCAGGTAGACTGTC | 21,055–21,025 | |
ADV-RP-nfo | FITC-CTCGATGACGCCGCGGTGTGGCTGGTGCAC[THF]CTGACCACGTCGAAGA-SpC3 | 20,979–21,025 | |
Real-time RPA | ADV-RF | GCTCATTGGAACAACTGCCGTAAATAGTGT | 20,761–20,790 |
ADV-RR | GGCCGAGAACGGTGTACGCAGGTAGACTGTC | 21,055–21,025 | |
ADV-RP-exo | CAGGTAGACTGTCTCGATGACGCCGCGGTGT[FAM]G[THF]CT[BHQ]GGTGCACGCTGACC-SpC3 | 20,989–21,037 |