Table 2 Mean percent difference in the relative phyla abundance in different storage conditions. Data is presented as mean ± SEM. Significant differences are highlighted in bold (p < 0.05)
From: Sample storage conditions induce post-collection biases in microbiome profiles
Phylum | Fresh | EDTA vs Fresh | Lysis vs Fresh | RT vs Fresh | 4 °C vs Fresh | − 20 °C vs Fresh | − 80 °C vs Fresh | HY vs Fresh |
|---|---|---|---|---|---|---|---|---|
Firmicutes | 68.42% ± 0.06% | − 12.17% ± 2.33% | −11.36% ± 4.97% | −18.57% ± 8.89% | −23.85% ± 0.87% | − 16.07% ± 0.73% | − 16.56% ± 5.64% | − 27.52% ± 2.02% |
Bacteroides | 22.14% ± 0.59% | + 15.52% ± 0.96% | + 2.14% ± 3.98% | + 21.72% ± 9.20% | + 27.06% ± 0.79% | + 18.01% ± 1.38% | + 18.38% ± 5.91% | + 32.7% ± 2.17% |
Actinobacteria | 6.00% ± 0.54% | − 2.36% ± 0.65% | 6.08% ± 0.11% | − 3.58% ± 0.77% | −4.29% ± 0.11% | −3.33% ± 0.27% | −2.84% ± 0.46% | −5.38% ± 0.04% |
Verrucomicrobia | 0.63% ± 0.01% | + 0.13% ± 0.31% | + 2.56% ± 0.74% | + 0.42% ± 0.08% | + 0.74% ± 0.25% | + 0.85% ± 0.41% | + 0.66% ± 0.11% | + 0.08% ± 0.01% |
Proteobacteria | 0.26% ± 0.03% | −0.03% ± 0.01% | 0.14% ± 0.17% | + 0.44% ±0.32% | + 0.78% ± 0.01% | + 0.50% ± 0.01% | + 0.45% ± 0.23% | + 0.58% ± 0.05% |
Tenericutes | 0.07% ± 0.01% | + 0.01% ± 0.03% | + 0.33% ± 0.02% | + 0.25% ± 0.13% | + 0.44% ± 0.06% | + 0.29% ± 0.11% | + 0.16% ± 0.03% | + 0.88% ± 0.07% |