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Table 2 Mean percent difference in the relative phyla abundance in different storage conditions. Data is presented as mean ± SEM. Significant differences are highlighted in bold (p < 0.05)

From: Sample storage conditions induce post-collection biases in microbiome profiles

Phylum Fresh EDTA vs Fresh Lysis vs Fresh RT vs Fresh 4 °C vs Fresh − 20 °C vs Fresh − 80 °C vs Fresh HY vs Fresh
Firmicutes 68.42% ± 0.06% − 12.17% ± 2.33% −11.36% ± 4.97% −18.57% ± 8.89% −23.85% ± 0.87% − 16.07% ± 0.73% − 16.56% ± 5.64% − 27.52% ± 2.02%
Bacteroides 22.14% ± 0.59% + 15.52% ± 0.96% + 2.14% ± 3.98% + 21.72% ± 9.20% + 27.06% ± 0.79% + 18.01% ± 1.38% + 18.38% ± 5.91% + 32.7% ± 2.17%
Actinobacteria 6.00% ± 0.54% − 2.36% ± 0.65% 6.08% ± 0.11% − 3.58% ± 0.77% −4.29% ± 0.11% −3.33% ± 0.27% −2.84% ± 0.46% −5.38% ± 0.04%
Verrucomicrobia 0.63% ± 0.01% + 0.13% ± 0.31% + 2.56% ± 0.74% + 0.42% ± 0.08% + 0.74% ± 0.25% + 0.85% ± 0.41% + 0.66% ± 0.11% + 0.08% ± 0.01%
Proteobacteria 0.26% ± 0.03% −0.03% ± 0.01% 0.14% ± 0.17% + 0.44% ±0.32% + 0.78% ± 0.01% + 0.50% ± 0.01% + 0.45% ± 0.23% + 0.58% ± 0.05%
Tenericutes 0.07% ± 0.01% + 0.01% ± 0.03% + 0.33% ± 0.02% + 0.25% ± 0.13% + 0.44% ± 0.06% + 0.29% ± 0.11% + 0.16% ± 0.03% + 0.88% ± 0.07%