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Table 1 Sample stabilization and storage conditions

From: Sample storage conditions induce post-collection biases in microbiome profiles

 

Classification

Time

Solution

Temperature

Oxygen levela

1.

Fresh

< 1 days

NA

NA

NA

2.

EDTA

33 days

100 mM EDTA

20 °C

Ambient

3.

Lysis

33 days

S1 and S2b

20 °C

Ambient

4.

RT

33 days

NA

20 °C

Ambient

5.

4 °C

33 days

NA

4 °C

Ambient

6.

−20 °C

33 days

NA

−20 °C

Ambient

7.

−80 °C

33 days

NA

−80 °C

Ambient

8.

Hypoxia

33 days

NA

20 °C

< 10 mmHg pO2

  1. aAmbient pO2 is anticipated to be 160 mmHg pO2 21% O2; 10 mmHg pO2 1.3% O2
  2. bProprietary. Obtained from PureLink Microbiome DNA Purification Kit (Invitrogen #A29790). NA = Not Applicable