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Table 1 Sample stabilization and storage conditions

From: Sample storage conditions induce post-collection biases in microbiome profiles

  Classification Time Solution Temperature Oxygen levela
1. Fresh < 1 days NA NA NA
2. EDTA 33 days 100 mM EDTA 20 °C Ambient
3. Lysis 33 days S1 and S2b 20 °C Ambient
4. RT 33 days NA 20 °C Ambient
5. 4 °C 33 days NA 4 °C Ambient
6. −20 °C 33 days NA −20 °C Ambient
7. −80 °C 33 days NA −80 °C Ambient
8. Hypoxia 33 days NA 20 °C < 10 mmHg pO2
  1. aAmbient pO2 is anticipated to be 160 mmHg pO2 21% O2; 10 mmHg pO2 1.3% O2
  2. bProprietary. Obtained from PureLink Microbiome DNA Purification Kit (Invitrogen #A29790). NA = Not Applicable