Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Table 3 List of oligonucleotide primer pairs and protocols designed for the detection of VAT genes in A. lanthieri strains

Target VAT Gene	Oligonucleotide Primer Sequences (5’-3’)	Amplicon size (bp)	Primer Concentration (μM)	Annealing Temperature (°C)
Assay 1
cdtB	cdtB F: GCA AAA GGT GAT TGG GCT CC	303	0.4	56
cdtB	cdtB R: TCC TCC AGC TCC TTG AAC AC	303	0.4
cadF	cadF F: TCC AAC TCC AGT TGC TGC TC	243	0.4
cadF	cadF R: TGT CCT TCG ATG TCA GCT TTC	243	0.4
irgA	irgA F: AGA GCT GTT GGT TGG GAT GG	186	0.4
irgA	irgA R: TGC ATT TGC TCT TGT AGG GT	186	0.4
Assay 2
cdtC	cdtC F: GAT GAA TCC ACC AGA AAT AGA G	196	0.3	57
cdtC	cdtC R: TTT GGG ATC AAG AGT ATA AAG TTC	196	0.3
pldA	pldA F: TGC TCC ATT TAG AGA AAC TAA C	132	0.1
pldA	pldA R: GAA CGA GAT TCT TCA CCA TCT T	132	0.1
Assay 3
cdtA	cdtA F: CAG GAA TAG ATC TCG CTA CAA ATG	220	0.3	55
cdtA	cdtA R: TTT GGT AGA AGA GGA AGT TCA TTG	220	0.3
mviN	mviN F: ACC TTT GGT TCT TCA ACT TTA C	170	0.4
mviN	mviN R: CGT GCT ACC ATA GGA AAT AGG	170	0.4
Assay 4
ciaB	ciaB F: GAT AGA TGC TAT TCT GCT CTT G	207	0.2	60
ciaB	ciaB R: ATC TTC ACT AAA TGC TAC TAT T	207	0.2
tylA	tylA F: GAC ATT GTA ACA TGT GAT GTA TCT T	125	0.1
tylA	tylA R: TTT ACA TTT GTT CCC ACT TCA AA	125	0.1
Assay 5
tet(O)	tetO F: TCA TAC ACT TAT ACA CTT GAT GC	94	0.2	62
tet(O)	tetO R: CTC TAT ATT CAT TTG CAA CAT CAA CT	94	0.2	62
Assay 6
tet(W)	tetW F: AAT CAT ACA CTT ATA CAC TTG ATG CAC	197	0.1	60
tet(W)	tetW R: TTT AAT TCC TTC AAC TAT CTC TTC TTC	197	0.1	60

ISSN: 1471-2180