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Table 3 List of oligonucleotide primer pairs and protocols designed for the detection of VAT genes in A. lanthieri strains

From: Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Target VAT Gene Oligonucleotide Primer Sequences (5’-3’) Amplicon size (bp) Primer Concentration (μM) Annealing Temperature (°C)
Assay 1
cdtB cdtB F: GCA AAA GGT GAT TGG GCT CC 303 0.4 56
cdtB R: TCC TCC AGC TCC TTG AAC AC 0.4
cadF cadF F: TCC AAC TCC AGT TGC TGC TC 243 0.4
cadF R: TGT CCT TCG ATG TCA GCT TTC 0.4
irgA irgA F: AGA GCT GTT GGT TGG GAT GG 186 0.4
irgA R: TGC ATT TGC TCT TGT AGG GT 0.4
Assay 2
cdtC cdtC F: GAT GAA TCC ACC AGA AAT AGA G 196 0.3 57
cdtC R: TTT GGG ATC AAG AGT ATA AAG TTC 0.3
pldA pldA F: TGC TCC ATT TAG AGA AAC TAA C 132 0.1
pldA R: GAA CGA GAT TCT TCA CCA TCT T 0.1
Assay 3
cdtA cdtA F: CAG GAA TAG ATC TCG CTA CAA ATG 220 0.3 55
cdtA R: TTT GGT AGA AGA GGA AGT TCA TTG 0.3
mviN mviN F: ACC TTT GGT TCT TCA ACT TTA C 170 0.4
mviN R: CGT GCT ACC ATA GGA AAT AGG 0.4
Assay 4
ciaB ciaB F: GAT AGA TGC TAT TCT GCT CTT G 207 0.2 60
ciaB R: ATC TTC ACT AAA TGC TAC TAT T 0.2
tylA tylA F: GAC ATT GTA ACA TGT GAT GTA TCT T 125 0.1
tylA R: TTT ACA TTT GTT CCC ACT TCA AA 0.1
Assay 5
tet(O) tetO F: TCA TAC ACT TAT ACA CTT GAT GC 94 0.2 62
tetO R: CTC TAT ATT CAT TTG CAA CAT CAA CT 0.2
Assay 6
tet(W) tetW F: AAT CAT ACA CTT ATA CAC TTG ATG CAC 197 0.1 60
tetW R: TTT AAT TCC TTC AAC TAT CTC TTC TTC 0.1