Skip to main content

Table 2 List of oligonucleotide primer pairs and PCR protocols designed and optimized for the detection of VAT genes in A. faecis

From: Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Target VAT Gene Oligonucleotide Primer Sequences (5’-3’) Amplicon Size (bp) Primer Concentration (μM) Annealing Temperature (°C)
Assay 1
mviN mviN F: TTC TTT GCA GCA ACA TTG GG 180 0.4 57
mviN R: TGC TAC CAT AGG AAA TAG GGC 0.4
irgA irgA F: CTG GAC AGT ATG AAG GAA ACC C 105 0.4
irgA R: TTG CTT GAG TCC ATA ACA ACC A 0.4
Assay 2
cadF cadF F: ATG GTG CAT TCG GAA ACT ACG G 192 0.1 60
cadF R: TTG GAG CTG GAG CAG GAA CTA 0.1
tylA tylA F: TAG AAA CAA AGC AAG TGA ACT C 116 0.2
tylA R: CCT CTT CAA GTA GCT CTA TAT T 0.2
Assay 3
cdtA cdtA F: TGT AGC CGA TGA ACT TAG TGA AGT AGA G 274 0.4 59
cdtA R: CCC AAC TGT TGC TTG TCC CAT TA 0.4
ciaB ciaB F: AAG CAG TTG CCC TAG AGT GG 196 0.4
ciaB R: AGT GCT GGT CGT CCA ACA TAA 0.4
pldA pldA F: GTG CTG CTG AAT TTA ACT GG 145 0.4
pldA R: GCA ACA CCT ATT CCT ACA TTT G 0.4
Assay 4
cdtC cdtC F: AAG CAG AGG GTG AAA TAG CC 210 0.8 56
cdtC R: GAT TAG CAA ACT GTC CAC CAA ATA C 0.8
tet(W) tet(W) F: ACA TCA TTG ATA CTC CAG GTC ACG 120 0.2
tet(W) R: TTT CAC TTT GTG GTT GAA CCC CTC 0.2
Assay 5
tet(O) tetO F: GGA GGG GTT CAA CCA C AA AG 88 0.1 54
tetO R: CTA TGT AAA TAA AAT GGA TAG 0.1