Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Table 2 List of oligonucleotide primer pairs and PCR protocols designed and optimized for the detection of VAT genes in A. faecis

Target VAT Gene	Oligonucleotide Primer Sequences (5’-3’)	Amplicon Size (bp)	Primer Concentration (μM)	Annealing Temperature (°C)
Assay 1
mviN	mviN F: TTC TTT GCA GCA ACA TTG GG	180	0.4	57
mviN	mviN R: TGC TAC CAT AGG AAA TAG GGC	180	0.4
irgA	irgA F: CTG GAC AGT ATG AAG GAA ACC C	105	0.4
irgA	irgA R: TTG CTT GAG TCC ATA ACA ACC A	105	0.4
Assay 2
cadF	cadF F: ATG GTG CAT TCG GAA ACT ACG G	192	0.1	60
cadF	cadF R: TTG GAG CTG GAG CAG GAA CTA	192	0.1
tylA	tylA F: TAG AAA CAA AGC AAG TGA ACT C	116	0.2
tylA	tylA R: CCT CTT CAA GTA GCT CTA TAT T	116	0.2
Assay 3
cdtA	cdtA F: TGT AGC CGA TGA ACT TAG TGA AGT AGA G	274	0.4	59
cdtA	cdtA R: CCC AAC TGT TGC TTG TCC CAT TA	274	0.4
ciaB	ciaB F: AAG CAG TTG CCC TAG AGT GG	196	0.4
ciaB	ciaB R: AGT GCT GGT CGT CCA ACA TAA	196	0.4
pldA	pldA F: GTG CTG CTG AAT TTA ACT GG	145	0.4
pldA	pldA R: GCA ACA CCT ATT CCT ACA TTT G	145	0.4
Assay 4
cdtC	cdtC F: AAG CAG AGG GTG AAA TAG CC	210	0.8	56
cdtC	cdtC R: GAT TAG CAA ACT GTC CAC CAA ATA C	210	0.8
tet(W)	tet(W) F: ACA TCA TTG ATA CTC CAG GTC ACG	120	0.2
tet(W)	tet(W) R: TTT CAC TTT GTG GTT GAA CCC CTC	120	0.2
Assay 5
tet(O)	tetO F: GGA GGG GTT CAA CCA C AA AG	88	0.1	54
tet(O)	tetO R: CTA TGT AAA TAA AAT GGA TAG	88	0.1	54

ISSN: 1471-2180