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Fig. 1 | BMC Microbiology

Fig. 1

From: Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Fig. 1

Typical amplicons for gene-specific mPCR assay developed for the detection of VAT genes in A. faecis. Panel a Lane 1: mviN (180 bp) and irgA (105 bp); and Lane 5: cadF (192 bp) and tylA (116 bp) genes; Panel b Lane 1: cdtA (274 bp), ciaB (196 bp) and pldA (145 bp); and Lane 5: cdtC (210 bp) and tet(W) (120 bp) and; Panel c Lane 1: tet(O) (88 bp) gene. Lanes 1: A. faecis LMG 28519 served as control positive; Lanes 2-4 and 6-8: A. butzleri ATCC 49616, A. cibarius LMG 21996, A. lanthieri LMG 28516 and Lanes 5 (Panel c), 9&10 (Panels a and b) served as control negative. Lane M: 1kb DNA marker

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