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Table 3 Strains and plasmids used in the study

From: Putrescine biosynthesis and export genes are essential for normal growth of avian pathogenic Escherichia coli

Strains and plasmids Relevant featuresa,b Reference
E. coli strains
E. coli (WT-ST117) Virulent reference strain, APEC strain [5]
 MG1655 MG1655/K-12- Reference strain ATCC 2592
E. coli K-12 ER2420 Cloning intermediate strain Termo Scientific
 ΔspeB-ST117 speB mutant, ChlR This work
 ΔspeC-ST117 speC mutant, ChlR This work
 ΔspeB/C-ST117 speB mutant, speC mutant, ChlR, TmpR This work
 ΔspeF-ST117 speF mutant, ChlR This work
 ΔspeD/E-ST117 speD mutant, speE mutant, ChlR This work
 ΔpotABCD-ST117 potABCD mutant, ChlR This work
 ΔpotFGHI-ST117 potFGHI mutant, ChlR This work
 ΔpotE-ST117 potE mutant, ChlR This work
 ΔpotE/pACYC184 potE mutant, Chl R/ potE gene, TetR This work
 ΔyeeF-ST117 yeeF mutant, ChlR This work
 ΔspeB-MG1655 speB mutant, ChlR This work
 ΔspeC-MG1655 speC mutant, ChlR This work
 ΔspeB/C-MG1655 speB mutant, speC mutant, ChlR, TmpR This work
 ΔspeF-MG1655 speF mutant, ChlR This work
 ΔspeD/E-MG1655 speD mutant, speE mutant, ChlR This work
 ΔpotABCD -MG1655 potABCD mutant, ChlR This work
 ΔpotFGHI -MG1655 potFGHI mutant, ChlR This work
 ΔpotE -MG1655 potE mutant, ChlR This work
 ΔpotF-MG1655 potE mutant, ChlR This work
Plasmids
 pKD46 Plasmid with λ-Red recombinase expressed from arabinose inducible promoter [39]
 pKD3 Template plasmid for λ -red mutagenesis, ChlR, AmpR Termo Scientific
 pJET 1.2 Cloning vector, AmpR Termo Scientific
 pACYC184 Expressing the ΔpotE gene, ChlR, Tet R Termo Scientific
 pM3224T Template plasmid for TmpR [44]
  1. aThe metabolic pathway affected for each mutant is indicated in Fig. 1
  2. bChlR chloramphenicol resistant; TetR tetracycline resistant; GenR gentamicin resistant; TmpR trimethoprim resistant; AmpR ampicillin resistant