Table 3 Bacterial strains and plasmids used in this study

 L. crescens BT-1

Standard strain

Leonard et al. (2012) [34]

 E. coli DH5α

φ80 dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk⁻. mk⁺) supE44 thi-1 gyrA relA1

Laboratory stock

 E. coli BL21 (DE3)

F− ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Life Technologies

 E. coli BW25113

Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ⁻ rph-1 Δ(rhaD-rhaB)568 hsdR514; (WT)

Kitagawa et al. (2005) [48]

 JC1001

E. coli BW25113 pJFC1; (Δldt5/ldtP⁺)

This study

 PM2405

E. coli BW25113 ΔynhG753::frt ΔybiS790::frt ΔycfS775::frt ΔerfK761::frt ΔycbB742::kan; (Δldt5)

Sanders & Pavelka (2013) [7]

 PM2405A

E. coli PM2405 pJFC1; (Δldt5/ldtP⁺)

This study

 PM2405A1

E. coli PM2405 pJFC1F; (Δldt5/ldtP⁺ -FLAG)

This study

 p15TV-L

Expression vector for purification of proteins by nickel affinity chromatography. Ap^R

Structural Genomics Consortium, University of Toronto

 pBAD24

Arabinose-inducible araBAD expression vector; optimized Shine-Dalgarno box (RBS), no purification tag in MCS; Ap^R

Guzman et al. (1995) [12]

 pJFC1

pBAD24 derivative containing ldtP from L. asiaticus (NcoI/AccI); Ap^R

This study

 pJFC1F

pBAD24 derivative containing ldtP from L. asiaticus with a C-terminal FLAG-tag (NcoI/AccI); Ap^R

This study