VirB10 vaccination for protection against Anaplasma phagocytophilum

BMC Microbiology

Table 1 Oligonucleotides used in this study

		Target
DNA vaccines
AB1656	GCCACCATGAGCACAAATATTGGCGTACC	virB9-1
AB1657	GACTAAGAGCCTGATTCACAACTTCTAC	virB9-1
AB1658	GCCACCATGGCTGATGATCACCATTAAGACC	virB9-2
AB1659	GTTTCCGGCGTCTTTCAGCACCCTTC	virB9-2
AB1660	GCCACCATGGCTGACGAAATAAGGGG	virB10
AB1661	GCCTCACCGCATCACGAGGAAATA	virB10
Recombinant proteins
AB1703	CACCATGAGCACAAATATTGGCGTACCAG	virB9-1
AB1704	ACTAAGAGCCTGATTCACAACTTCTACACTCCTGC	virB9-1
AB1705	CACCATGGCTGATGATCACATTAAGACCTTGAAC	virB9-2
AB1706	TTTCCGGCGTCTTTCAGCACCCTTC	virB9-2
AB1707	CACCATGGCTGACGAAATAAGGGGTTCTAG	virB10
AB1708	CCTCACCGCATCACGAGGAAATACTACG	virB10
qPCR
AB1334	AGATGCTGACTGGGGATGAG	msp5
AB1335	TCGGCATCAACCAAGTACAA
^aAB1336	CGTAGGTGAGTCTGATAGTGAAGG

Underlined letters correspond to vector nucleotides added to enable directional cloning
^a Probe labeled with Hexachloro-fluorescein (HEX) at the 5′ end

ISSN: 1471-2180