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Table 2 Clearing Zone Index for the Production of α-Amylases by A. oryzae cmc1 Mutants on Agar Plates containing 2-Deoxy D-Glucose (0.1%, w/v) & 0.2% Triton X100

From: Random mutagenesis of super Koji (Aspergillus oryzae): improvement in production and thermal stability of α-amylases for maltose syrup production

Sr. No Mutant no. Halo zone + Colony diameter (cm) Colony diameter (cm) Clearing zone index
1 Parent Koji 1.2±0.07 0.60±0.04 2.00
2 M-60(2) 1.3±0.08 0.50±0.03 2.60
3 M-60(4) 1.1±0.06 0.60±0.04 1.83
4 M-60(5) 1.8±0.14 0.65±0.04 2.77
5 M-60(7) 0.8±0.06 0.70±0.04 1.14
6 M-60(10) 1.1±0.08 0.60±0.03 1.83
7 M-60(11) 0.9±0.06 0.60±0.04 1.50
8 M-80(6) 1.3±0.05 0.60±0.03 2.16
9 M-80(9) 1.2±0.05 0.50±0.03 2.40
10 M-80(10) 1.7±0.08 0.55±0.03 3.09
11 M-80(11) 1.5±0.06 0.60±0.04 2.50
12 M-80(12) 1.6±0.07 0.60±0.03 2.66
13 M-100(2) 1.4±0.06 0.70±0.04 2.00
14 M-100(3) 1.5±0.08 0.65±0.04 2.30
15 M-100(4) 0.9±0.04 0.40±0.02 2.25
16 M-100(6) 1.9±0.09 0.60±0.04 3.16
17 M-100(7) 1.7±0.08 0.70±0.04 2.42
18 M-100(9) 1.1±0.05 0.50±0.03 2.20
19 M-100(12) 1.9±0.09 0.65±0.04 2.92
20 M-120(4) 1.3±0.08 0.60±0.03 2.16
21 M-120(5) 1.7±0.10 0.50±0.03 3.40
22 M-120(10) 1.3±0.07 0.55±0.03 2.36
23 M-120(11) 1.6±0.09 0.60±0.03 2.67
24 M-140(6) 1.6±0.09 0.60±0.04 2.67
  1. Clearance zone index (CI) = (halo zone diameter + colony diameter) /colony diameter. Data presented are average values ± SD of n = 3 experiments