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Table 2 Clearing Zone Index for the Production of α-Amylases by A. oryzae cmc1 Mutants on Agar Plates containing 2-Deoxy D-Glucose (0.1%, w/v) & 0.2% Triton X100

From: Random mutagenesis of super Koji (Aspergillus oryzae): improvement in production and thermal stability of α-amylases for maltose syrup production

Sr. No

Mutant no.

Halo zone + Colony diameter (cm)

Colony diameter (cm)

Clearing zone index

1

Parent Koji

1.2±0.07

0.60±0.04

2.00

2

M-60(2)

1.3±0.08

0.50±0.03

2.60

3

M-60(4)

1.1±0.06

0.60±0.04

1.83

4

M-60(5)

1.8±0.14

0.65±0.04

2.77

5

M-60(7)

0.8±0.06

0.70±0.04

1.14

6

M-60(10)

1.1±0.08

0.60±0.03

1.83

7

M-60(11)

0.9±0.06

0.60±0.04

1.50

8

M-80(6)

1.3±0.05

0.60±0.03

2.16

9

M-80(9)

1.2±0.05

0.50±0.03

2.40

10

M-80(10)

1.7±0.08

0.55±0.03

3.09

11

M-80(11)

1.5±0.06

0.60±0.04

2.50

12

M-80(12)

1.6±0.07

0.60±0.03

2.66

13

M-100(2)

1.4±0.06

0.70±0.04

2.00

14

M-100(3)

1.5±0.08

0.65±0.04

2.30

15

M-100(4)

0.9±0.04

0.40±0.02

2.25

16

M-100(6)

1.9±0.09

0.60±0.04

3.16

17

M-100(7)

1.7±0.08

0.70±0.04

2.42

18

M-100(9)

1.1±0.05

0.50±0.03

2.20

19

M-100(12)

1.9±0.09

0.65±0.04

2.92

20

M-120(4)

1.3±0.08

0.60±0.03

2.16

21

M-120(5)

1.7±0.10

0.50±0.03

3.40

22

M-120(10)

1.3±0.07

0.55±0.03

2.36

23

M-120(11)

1.6±0.09

0.60±0.03

2.67

24

M-140(6)

1.6±0.09

0.60±0.04

2.67

  1. Clearance zone index (CI) = (halo zone diameter + colony diameter) /colony diameter. Data presented are average values ± SD of n = 3 experiments