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Fig. 5 | BMC Microbiology

Fig. 5

From: Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding

Fig. 5

Results of DNA metabarcoding of field samples and comparisons with shotgun whole-genome metagenomics (WGS). a Stacked barplots showing the relative abundance of the genera detected by WGS and DNA metabarcoding with GTAA, BITS, and SP primers. The 28 samples were collected from mature vines in the field with the following trunk disease symptoms: apparently healthy (no foliar or wood symptoms), Eutypa Dieback, Esca and wood cankers (no leaf symptoms). The numbering corresponds to different biological replicates grouped by the disease symptoms displayed by the grapevines from where they were collected. b Scatter plots showing the correlations of the relative abundance obtained by DNA metabarcoding and WGS. c Scatter plots showing the correlations of the relative abundance separately for each genus obtained by DNA metabarcoding using the GTAA primers and WGS. d Scatter plots showing the correlations of the relative abundance separately for each genus obtained by DNA metabarcoding using the BITS primers and WGS. R values correspond to Pearson’s correlation coefficients

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