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Table 4 Glycopeptide MICs and detected mutations in vanB gene cluster of the VAN-resistant revertants

From: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

Strain

Mutationa

MIC (mg/L)b

RT-PCR (Fold change (±SE)c

vanSB gene

vanB gene

VAN

TEC

VAN (−)

VAN (+)

M1TC

–

–

3

1

N. D.

1

M1TCR1

N. D.

N. D.

16

1

N. D.

4.4 (±1.0)

M1TCR2

N. D.

574G > A(Ala192Thr)

16

1

N. D.

3.6 (±0.5)

M1TCR3

661G > A(Glu221Lys)

N. D.

16

1

N. D.

5.8 (±1.2)

M1TCR4

N. D.

N. D.

16

1

–

–

M1TCR5

N. D.

N. D.

16

1

N. D.

4.9 (±1.4)

M1TCR6

648-649 ins CTGGAGGATGAAA

TCGCAAGG

N. D.

128

128

36.1 (±8.0)

39.5 (±13.9)

M1TCR7

1200–1217 del

N. D.

16

1

–

–

M1TCR8

N. D.

N. D.

16

1

–

–

M1TCR9

N. D.

N. D.

16

1

–

–

M1TCR10

661G > A(Glu221Lys)

N. D.

32

1

–

–

Y7TC

–

–

3

1

  

Y7TCR1

722C > T(Ala241Val)

N. D.

16

1

–

–

Y7TCR2

N. D.

N. D.

16

1

–

–

Y7TCR3

574G > A(Ala192Thr)

N. D.

64

1

–

–

Y7TCR4

1218G > C(Gly406Ala)

317C > A(Ala106Glu)

16

1

–

–

  1. aThe sequence was compared with the vanB gene cluster of the cognate parent strain, M1TC or Y7TC. bVAN: vancomycin, TEC: teicoplanin. cReal-time PCR data represent the fold changes in vanXB transcriptional level relative to that of M1TC in the presence of vancomycin (1 mg/L; VAN (+)). The values were the means of three independent experiments with standard error, each experiment performed in duplicate. N. D.; not detected