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Table 4 Glycopeptide MICs and detected mutations in vanB gene cluster of the VAN-resistant revertants

From: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

Strain Mutationa MIC (mg/L)b RT-PCR (Fold change (±SE)c
vanSB gene vanB gene VAN TEC VAN (−) VAN (+)
M1TC 3 1 N. D. 1
M1TCR1 N. D. N. D. 16 1 N. D. 4.4 (±1.0)
M1TCR2 N. D. 574G > A(Ala192Thr) 16 1 N. D. 3.6 (±0.5)
M1TCR3 661G > A(Glu221Lys) N. D. 16 1 N. D. 5.8 (±1.2)
M1TCR4 N. D. N. D. 16 1
M1TCR5 N. D. N. D. 16 1 N. D. 4.9 (±1.4)
M1TCR6 648-649 ins CTGGAGGATGAAA
TCGCAAGG
N. D. 128 128 36.1 (±8.0) 39.5 (±13.9)
M1TCR7 1200–1217 del N. D. 16 1
M1TCR8 N. D. N. D. 16 1
M1TCR9 N. D. N. D. 16 1
M1TCR10 661G > A(Glu221Lys) N. D. 32 1
Y7TC 3 1   
Y7TCR1 722C > T(Ala241Val) N. D. 16 1
Y7TCR2 N. D. N. D. 16 1
Y7TCR3 574G > A(Ala192Thr) N. D. 64 1
Y7TCR4 1218G > C(Gly406Ala) 317C > A(Ala106Glu) 16 1
  1. aThe sequence was compared with the vanB gene cluster of the cognate parent strain, M1TC or Y7TC. bVAN: vancomycin, TEC: teicoplanin. cReal-time PCR data represent the fold changes in vanXB transcriptional level relative to that of M1TC in the presence of vancomycin (1 mg/L; VAN (+)). The values were the means of three independent experiments with standard error, each experiment performed in duplicate. N. D.; not detected