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Table 1 Bacterial strains used in this study

From: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

Strain/plasmid

Species

Source

Date of Isolation (mo/yr)

Hospital

VRE

genotype

Multilocus sequence typing

PFGE

types

MIC (mg/L)a

VAN

TEC

AMP

GEN

KAN

STR

CHL

TET

ERY

CIP

M1

E. faecium

feces

09/09

A

VanB

ST78

I-C

3

1

256

> 1024

> 1024

32

8

64

>256

64

M2

E. faecium

feces

10/09

A

VanB

ST78

I-C

3

1

256

> 1024

> 1024

32

8

64

>256

64

M3

E. faecium

feces

10/09

A

VanB

ST78

I-A

3

1

256

> 1024

> 1024

32

8

64

>256

32

M4

E. faecium

feces

10/09

A

VanB

ST78

I-B

3

1

256

8

> 1024

32

8

64

>256

64

M5

E. faecium

feces

10/09

A

VanB

ST78

III

3

1

128

8

128

256

8

64

>256

64

M6

E. faecium

feces

10/09

A

VanB

ST78

II

3

1

256

8

> 1024

32

8

64

>256

64

M7

E. faecium

feces

10/09

A

VanB

ST78

I-C

3

1

256

> 1024

> 1024

32

8

64

>256

64

M8

E. faecium

feces

10/09

A

VanB

ST78

I-A

3

1

256

> 1024

> 1024

32

8

64

>256

64

M9

E. faecium

feces

10/09

A

VanB

ST78

III

3

1

128

8

128

256

8

64

>256

64

M10

E. faecium

feces

0/09

A

VanB

ST78

I-A

3

1

256

> 1024

> 102

32

8

64

>256

64

Y7

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y8

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y9

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y10

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y11

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y12

E. faecium

feces

02/10

B

VanB

ST78

II

3

1

128

> 1024

> 1024

32

8

64

>256

64

Y13

E. faecium

feces

03/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y14

E. faecium

feces

03/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

Y15

E. faecium

feces

03/10

B

VanB

ST78

II

3

1

256

> 1024

> 1024

32

8

64

>256

64

FA2–2

E. faecalis

–

–

–

–

–

–

1

1

2

32

128

64

8

2

0.5

1

FA2–2/pMG2200

E. faecalis

–

–

–

VanB

–

–

64

1

2

32

128

64

8

2

0.5

1

M1TCb

E. faecalis

–

–

–

VanB

–

–

3

1

2

> 1024

> 1024

64

8

64

0.5

1

Y1TCb

E. faecalis

–

–

–

VanB

–

–

3

1

2

> 1024

> 1024

64

8

64

0.5

1

BM4105RF

E. faecium

–

–

–

–

–

–

1

1

3

8

> 1024

32

2

1

0.5

2

M1TC2c

E. faecium

–

–

–

VanB

–

–

3

1

3

> 1024

> 1024

32

2

128

0.5

2

Y7TC2c

E. faecium

–

–

–

VanB

–

–

3

1

3

> 1024

> 1024

32

2

128

0.5

2

  1. aVAN, vancomycin; TEC, teicoplanin; AMP, ampicillin; GEN, gentamicin; KAN, kanamycin; STR, streptomycin; CHL, chloramphenicol; TET, tetracycline; ERY, erythromycin; CIP, ciprofloxacin. bCorresponding transconjugants of FA2–2 obtained by filter mating with the donor M1 or Y7 strain, respectively. cCorresponding transconjugants of BM4105RF obtained by filter mating with the donor M1 or Y7 strain, respectively