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Fig. 2 | BMC Microbiology

Fig. 2

From: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

Fig. 2

PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with SmaI and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

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