|
Treatment
|
% Relative Antibacterial Activity
|
Treatment
|
Antibacterial Activity (AU ml− 1)
|
|---|
|
Control
|
100
|
Control
|
80
|
|
a. Temperature (°C)
|
Exposure Time (hours)
|
|
1.0
|
2.0
|
3.0
|
4.0
|
d. Surfactants
b
|
|
40
|
100
|
100
|
100
|
100
|
|
60
|
100
|
100
|
100
|
96
|
Tween 20 (10 mM)
|
160
|
|
80
|
97
|
83
|
80
|
75
|
Tween 80 (10 mM)
|
160
|
|
100
|
70
|
50
|
nil
|
nil
|
EDTA (100 mM)
|
160
|
|
b. pH
|
Exposure Time (minutes)
|
SDS (01 mM)
|
160
|
|
15.0
|
30.0
|
60.0
|
120.0
|
Triton X-100 (0.25 mM)
|
nil
|
|
5.0
|
nil
|
nd
|
nd
|
nd
|
e. Organic Solvents
c
|
|
6.0
|
60
|
60
|
60
|
60
|
|
7.0
|
100
|
100
|
100
|
100
|
DMSO (100 mM)
|
160
|
|
8.0
|
80
|
80
|
80
|
80
|
Ethanol (05 mM)
|
160
|
|
9.0
|
75
|
75
|
75
|
75
|
Methanol (100 mM)
|
80
|
|
c. Metal Ions
a
|
Concentration (mM)
|
Formaldehyde (20 mM)
|
80
|
|
1.0
|
5.0
|
10.0
|
Chloroform (100 mM)
|
40
|
|
Ba+ 2
|
100
|
100
|
100
|
Isopropanol (100 mM)
|
20
|
|
Cu+ 2
|
100
|
nil
|
nd
|
f. Enzymes
d
|
37 °C
|
50 °C
|
|
Mn+ 2
|
100
|
90
|
90
|
02 h
|
24 h
|
02 h
|
24 h
|
|
Cs+ 2
|
100
|
90
|
90
|
Protease (0.5 U)
|
80
|
40
|
80
|
40
|
|
Ca+ 2
|
100
|
100
|
100
|
Proteinase K (20.0 U)
|
80
|
40
|
80
|
40
|
|
Mg+ 2
|
95
|
95
|
90
|
Pepsin (15.0 U)
|
80
|
40
|
80
|
40
|
|
Ni+ 2
|
80
|
80
|
80
|
Lysozyme (150,000 U)
|
40
|
nil
|
40
|
nil
|
|
Zn+ 2
|
70
|
30
|
nil
|
Amylase (30.0 U)
|
40
|
nil
|
40
|
nil
|
|
Co+ 2
|
60
|
nil
|
nd
|
Ribonuclease A (90.0 U)
|
80
|
80
|
80
|
80
|
|
Hg+ 2
|
nil
|
nd
|
nd
|
Catalase (11,000 U)
|
80
|
80
|
80
|
80
|
- nd Not determined
- nil No antibacterial activity
- aMetal ions used as chloride salts
- bNone of the surfactants alone (−ve control) in the above mentioned concentration inhibited the indicator strain except Triton X-100
- cNone of the organic solvents alone (−ve control) in the above mentioned concentration inhibited the indicator strain
- dFinal Concentration of each enzyme: 1.0 mg
