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Table 2 Antibacterial activity of BAC-IB17 against methicillin resistant Staphylococcus aureus after treatment with various physical and chemical parameters

From: Screening, purification and characterization of thermostable, protease resistant Bacteriocin active against methicillin resistant Staphylococcus aureus (MRSA)

Treatment % Relative Antibacterial Activity Treatment Antibacterial Activity (AU ml− 1)
Control 100 Control 80
a. Temperature (°C) Exposure Time (hours)
1.0 2.0 3.0 4.0 d. Surfactants b
40 100 100 100 100
60 100 100 100 96 Tween 20 (10 mM) 160
80 97 83 80 75 Tween 80 (10 mM) 160
100 70 50 nil nil EDTA (100 mM) 160
b. pH Exposure Time (minutes) SDS (01 mM) 160
15.0 30.0 60.0 120.0 Triton X-100 (0.25 mM) nil
5.0 nil nd nd nd e. Organic Solvents c
6.0 60 60 60 60
7.0 100 100 100 100 DMSO (100 mM) 160
8.0 80 80 80 80 Ethanol (05 mM) 160
9.0 75 75 75 75 Methanol (100 mM) 80
c. Metal Ions a Concentration (mM) Formaldehyde (20 mM) 80
1.0 5.0 10.0 Chloroform (100 mM) 40
Ba+ 2 100 100 100 Isopropanol (100 mM) 20
Cu+ 2 100 nil nd f. Enzymes d 37 °C 50 °C
Mn+ 2 100 90 90 02 h 24 h 02 h 24 h
Cs+ 2 100 90 90 Protease (0.5 U) 80 40 80 40
Ca+ 2 100 100 100 Proteinase K (20.0 U) 80 40 80 40
Mg+ 2 95 95 90 Pepsin (15.0 U) 80 40 80 40
Ni+ 2 80 80 80 Lysozyme (150,000 U) 40 nil 40 nil
Zn+ 2 70 30 nil Amylase (30.0 U) 40 nil 40 nil
Co+ 2 60 nil nd Ribonuclease A (90.0 U) 80 80 80 80
Hg+ 2 nil nd nd Catalase (11,000 U) 80 80 80 80
  1. nd Not determined
  2. nil No antibacterial activity
  3. aMetal ions used as chloride salts
  4. bNone of the surfactants alone (−ve control) in the above mentioned concentration inhibited the indicator strain except Triton X-100
  5. cNone of the organic solvents alone (−ve control) in the above mentioned concentration inhibited the indicator strain
  6. dFinal Concentration of each enzyme: 1.0 mg