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Fig. 2 | BMC Microbiology

Fig. 2

From: Screening, purification and characterization of thermostable, protease resistant Bacteriocin active against methicillin resistant Staphylococcus aureus (MRSA)

Fig. 2

a SDS-PAGE Profile of BAC-IB17 produced by Bacillus subtilis KIBGE-IB17. Lane M: Low molecular weight markers (6500-66,000, Sigma-Aldrich, USA). Lane 1: BAC-IB17 precipitates after (NH4)2SO4 saturation. Lane 2: BAC-IB17 precipitates after passing through PD-10 desalting column. Lane 3: BAC-IB17 precipitates after passing through Centricon® ultrafilter device. Lane 4: Purified and concentrated BAC-IB 17 after passing through Sepahrose-CL6B column. b Gel Overlay Assay of Band of interest against MRSA

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