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Table 1 The concentration and purity of DNA extracted using different methods and followed by MDA using REPLI-g® UltraFast Mini kit

From: A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides

Samples DNA extraction Pre-MDA Post-MDA
Undiluted 1:25 dilution in ddH2O
Purity (260/280 ratio) Concentration (ng/μl) Purity (260/280 ratio) Concentration (ng/μl) Purity (260/280 ratio) Concentration (ng/μl)
LCM 1 Heat shock 1.52 4.5 1.81 666.1 1.69 31.6
LCM 2 Heat shock followed by ethanol precipitation 1.55 2.3 1.80 730.4 1.65 52.3
LCM 3 QiaAmp DNA Micro kit (Qiagen) 1.58 3.6 1.81 765.1 1.60 32.5
Positive control Heat-shock from single colony 1.61 72.3 1.89 876.4 1.68 46.8