Samples | DNA extraction | Pre-MDA | Post-MDA | ||||
---|---|---|---|---|---|---|---|
Undiluted | 1:25 dilution in ddH2O | ||||||
Purity (260/280 ratio) | Concentration (ng/μl) | Purity (260/280 ratio) | Concentration (ng/μl) | Purity (260/280 ratio) | Concentration (ng/μl) | ||
LCM 1 | Heat shock | 1.52 | 4.5 | 1.81 | 666.1 | 1.69 | 31.6 |
LCM 2 | Heat shock followed by ethanol precipitation | 1.55 | 2.3 | 1.80 | 730.4 | 1.65 | 52.3 |
LCM 3 | QiaAmp DNA Micro kit (Qiagen) | 1.58 | 3.6 | 1.81 | 765.1 | 1.60 | 32.5 |
Positive control | Heat-shock from single colony | 1.61 | 72.3 | 1.89 | 876.4 | 1.68 | 46.8 |