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Table 1 The concentration and purity of DNA extracted using different methods and followed by MDA using REPLI-g® UltraFast Mini kit

From: A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides

Samples

DNA extraction

Pre-MDA

Post-MDA

Undiluted

1:25 dilution in ddH2O

Purity (260/280 ratio)

Concentration (ng/μl)

Purity (260/280 ratio)

Concentration (ng/μl)

Purity (260/280 ratio)

Concentration (ng/μl)

LCM 1

Heat shock

1.52

4.5

1.81

666.1

1.69

31.6

LCM 2

Heat shock followed by ethanol precipitation

1.55

2.3

1.80

730.4

1.65

52.3

LCM 3

QiaAmp DNA Micro kit (Qiagen)

1.58

3.6

1.81

765.1

1.60

32.5

Positive control

Heat-shock from single colony

1.61

72.3

1.89

876.4

1.68

46.8