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Fig. 2 | BMC Microbiology

Fig. 2

From: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

Fig. 2

Taxonomic composition of three environmental samples split into fungal and co-amplified sequences. Barchart indicates relative sequence abundance of the different a eukaryotic groups, and b fungal classes amplified by the primer pair nu-SSU-1333-5′/nu-SSU-1647-3′ (FF390/FR-1) after rarefying sequences. Two libraries were prepared on the same template DNA: (i) solely with the primer pair, and (ii) primer pair and four different blocking oligonucleotides designed for Stramenopiles, Alveolata, Rhizaria and Telonema (BO). Others in a: Centrohelida, Choanoflagellida, Corallochytrea, Discicristoidea, Excavata, Freshwater Opisthokonta, Filasterea, Picozoa, Telonema. Others in b: Pezizomycotina incertae sedis, Lecanoromycetes, Lichinomycetes, Pezizomycetes, Agaricomycetes, Cystobasidiomycetes, Agaricostilbomycetes, Microbotryomycetes, Pucciniomycetes, Wallemiomycetes, Pucciniomycotina incertae sedis, Ustilaginomycetes, LKM15

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