Table 2 The cps1 gene cluster of K. oxytoca DSM 29614
Gene (Locus tag) | Start/Stop nucleotides | Encoded Protein | |||
|---|---|---|---|---|---|
id | Function | % of Identitya | Comment | ||
BI322_RS06885 | 549463/550359 | WP_024360046.1 | UTP-glucose-1-phosphate uridylyltransferase (GalF) | 99 | |
BI322_RS06890 | 550820/551449 | WP_082236967.1 | acid phosphatase (phosphatidic acid phosphatase) | 98 | |
BI322_RS06895 | 552089/552316 | WP_082236968.1 | hypothetical protein | 0 | |
BI322_RS06900 | 552447/553886 | WP_082236969.1 | capsule assembly Wzi family protein (Wzi) | 99 | |
BI322_RS06905 | 553960/555096 | WP_082236970.1 | polysaccharide export protein (Wza) | 94 | Wza is required for the translocation of capsular polysaccharide through the outer membrane. |
BI322_RS06910 | 555098/555538 | WP_082236971.1 | protein tyrosine phosphatase (Wzb) | 88 | Wzb shows phosphatase activity towards the autophosphorylated Wzc protein, which induces colanic acid biosynthesis; catalyzes the phosphorylation of UDP-glucose dehydrogenase, an enzyme involved in colanic acid biosynthesis. |
BI322_RS06915 | 555549/557714 | WP_082236972.1 | tyrosine-protein kinase (Wzc) | 78 | Wzc catalyzes the autophosphorylation on tyrosine residues which downregulates the biosynthesis of colonic acid (an extracellular polysaccharide). |
BI322_RS06920 | 557836/559263 | WP_082236973.1 | undecaprenyl-phosphate galactose phosphotransferase (WbaP) | 0 | |
BI322_RS06925 | 559308/560219 | WP_082236974.1 | rhamnosyltransferase | 0 | |
BI322_RS06930 | 560570/561598 | WP_082236975.1 | hypothetical protein | 31 | |
BI322_RS06935 | 561614/562714 | WP_082236976.1 | EpsG family protein | 36 | This family of proteins are related to the EpsG protein from Bacillus subtilis. These proteins are likely glycosyl transferases belonging to the membrane protein GT-C clan. |
BI322_RS06940 | 562727/563569 | WP_082236977.1 | hypothetical protein | 44 | Glycosyltransferase like family 2; Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis. |
BI322_RS06945 | 563574/564449 | WP_082236978.1 | hypothetical protein | 99 | Glycosyltransferase, GT2 family, putative dTDP-rhamnosyl transferase. |
BI322_RS06950 | 564469/565359 | WP_082236979.1 | hypothetical protein | 0 | Glycosyltransferase like family 2; Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis. |
BI322_RS06955 | 565408/566238 | WP_082236980.1 | hypothetical protein | 36 | |
BI322_RS06960 | 566247/567692 | WP_082236981.1 | lipopolysaccharide biosynthesis protein (Wzx) | 50 | Membrane protein involved in the export of O-antigen and teichoic acid. |
BI322_RS06965 | 568041/569447 | WP_082236982.1 | NADP-dependent phosphogluconate dehydrogenase (Gnd) | 99 | 6-phosphogluconate dehydrogenase. |
BI322_RS06970 | 569654/570718 | WP_082236983.1 | dTDP-glucose 4,6-dehydratase | 99 | |
BI322_RS06975 | 570732/571601 | WP_049099678.1 | glucose-1-phosphate thymidylyltransferase | 99 | |
BI322_RS06980 | 571633/572523 | WP_082236984.1 | dTDP-4-dehydrorhamnose reductase | 99 | |
BI322_RS06985 | 572539/573093 | WP_014230057.1 | dTDP-4-dehydrorhamnose 3,5-epimerase | 99 | |
BI322_RS06990 | 573267/574433 | WP_082236985.1 | UDP-glucose 6-dehydrogenase (Ugd) | 99 | |
BI322_RS06995 | 57599/576603 | WP_082236986.1 | NAD-dependent epimerase | 99 | |