Table 2 Minimal biofilm inhibitory and eradication concentrations (MBIC and MBEC) determined by resazurin and cell counting methods
Bacterial strain | Antibiotic | MICa (μg/mL) | MBIC (μg/mL) | MBEC (μg/mL) | ||
|---|---|---|---|---|---|---|
Resazurin | CFU/mLb | Resazurin | CFU/mLb | |||
(decrease in RFU) | (growth inhibition) | (decrease in RFU) | (log10 reduction) | |||
Staphylococcus aureus ATCC 29213 | Ciprofloxacin | 0.5 | 1 (99%) | 1 (100%) | > 160 (18%) | > 160 (1.2) |
Staphylococcus aureus MRSA ATCC 43300 | Ciprofloxacin | 0.5 | 1 (99%) | 1 (100%) | > 160 (30%) | > 160 (1.7) |
Enterococcus faecalis ATCC 29212 | Ciprofloxacin | 1 | 1 (90%) | 1 (100%) | > 160 (35%) | > 160 (4.0) |
Enterococcus faecalis VRE ATCC 51575 | Ciprofloxacin | 0.5 | 1 (99%) | 1 (100%) | > 160 (0%) | > 160 (3.3) |
Enterococcus faecium ATCC 35667 | Linezolid | 4 | 2 (96%) | 2 (100%) | > 80 (26%) | > 80 (1.5) |
Enterococcus faecium VRE ATCC 70021 | Linezolid | 2 | 2 (99%) | 2 (97%) | > 80 (26%) | > 80 (1.8) |