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Fig. 1 | BMC Microbiology

Fig. 1

From: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

Fig. 1

Assessment of biofilm biomass by crystal violet staining and cell enumeration by colony count. Biofilm production analysis through crystal violet staining (OD 570 nm, bars) and cell enumeration (log10CFU/mL, lines) of six bacterial strains: (a) Staphylococcus aureus ATCC 29213, (b) Staphylococcus aureus MRSA ATCC 43300, (c) Enterococcus faecium ATCC 35667, (d) Enterococcus faecium VRE ATCC 700221, (e) Enterococcus faecalis ATCC 29212 and (f) Enterococcus faecalis VRE ATCC 51575, incubated in six different media: Mueller Hinton (MH), Tryptic Soy (TS), Tryptic Soy supplemented with 1% glucose (TSG), Tryptic Soy supplemented with 2% glucose (TS2G), Brain Heart Infusion (BHI) and Brain Heart Infusion supplemented with 1% glucose (BHIG). Error bars represent standard deviation of two independent experiments in triplicate. Lowercase letters indicate significant differences amongst media used for biofilm production. Similar letters denote no significant difference (P > 0.05)

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