Fig. 3

Many enzyme functions correlate with temperature. a A sensitivity plot showing the number of significant EC numbers (corrected p-value < 0.01) obtained at different cutoffs for minimum number of UniProt entries per organism. The gray dashed line indicates the selected cutoff. b Enzyme annotations as a proportion of the proteome. Each point represents the mean for all organisms growing at a given temperature. The means for archaea and bacteria were calculated separately. c Heat-maps showing how the occurrence of significant EC numbers change with growth temperatures in archaea. Each row represents a different EC number and each column a different growth temperature. All included EC numbers are statistically correlated (corrected p-value < 0.01) either positively (red) or negatively (blue) with growth temperature and a minimum phylogenetic distance score of six (see Methods for details). For each reported growth temperature, the ratio of organisms containing each specific EC annotation is shown by the intensity of the color. Gray indicates temperatures for which no growth temperatures are available in the dataset. d Analysis and color scale as in c, but performed with data from bacteria. r indicates Spearman’s correlation coefficient