Fig. 4
From: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
Generation of a P. aeruginosa insertion library with pSNC-mTn5ME. a Diagram of pSNC-mTn5ME, a derivative of pSNC-mTn5 that has MEs instead of OE and IE at the termini of mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. Results were average of three independent experiments, and bars represent mean ± SD (*p < 0.0001 and **p = 0.0004 by unpaired t-test). Colony restreaking and PCR assays are shown in Additional file 4: Figure S4. c Plasmid and transposon retention frequencies in A. baylyi. Results were average of three independent experiments, and bars represent mean ± SD (*p = 0.0029 and **p = 0.0006 by unpaired t-test). Colony restreaking and PCR assays are shown in Additional file 5: Figure S5. d Plasmid and transposon retention frequencies in P. aeruginosa PAO1. Results were average of three independent experiments, and bars represent mean ± SD (*p < 0.0001 and **p = 0.0065 by unpaired t-test). Colony restreaking and PCR assays are shown in Additional file 6: Figure S6. e Plasmid and transposon retention frequencies in the P. aeruginosa PAO1 mutant library generated with pSNC-mTn5ME. f Colony restreaking. 100/100 SucRKanR colonies of the mTn5ME library of P. aeruginosa were found to be KanRCamS. 50 are shown here. g Colony PCR of ten restreaked clones in (f) with the indicated primers. All were mTn5-positive and plasmid-negative. h Transposon insertion sites of 46 mutant clones from the mTn5ME insertion library of P. aeruginosa. Identical clones are shown only once, with their duplication numbers indicated in parenthesis