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Table 3 PhaR-activated genes associated with a PhaR-binding consensus sequence

From: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation

Genes or orf

PhaR binding site

USDA110 RPKM

ΔphaR RPKM

Fold changea

Description

phbB2 (bll0225)

TGCGCTGCACAC

154.34

74.52

2.07

acetoacetyl CoA reductase

ragA (bll0304)

TGCGACGCCGCA

8.95

2.29

3.91

two-component response regulator

blr0305

TGCGACGCCGCA

45.61

16.48

2.77

unknown protein

bll0805

CGCAACGCACAA

185.17

76.29

2.43

hypothetical protein

blr0806

CGCAACGCACAA

52.09

23.58

2.21

hypothetical protein

bll1416

TGCGCGGCAGCA

52.49

20.56

2.55

unknown protein

blr2204

TGCAGTCCAGAA

19.07

9.05

2.11

transcriptional regulatory protein AraC family

bll2446

TTCGCCGCAGAA

275.72

129.25

2.13

hypothetical protein

bsr2601

TGCACCGCAGCC

32.44

15.65

2.07

unknown protein

blr2810

TGCATTGCGCAA

15.88

7.35

2.16

aldo/keto reductase

bll2914

TGCGCTGGAGAA

25.07

9.61

2.61

probable amidase

bll3387

TGCGCCGCAACA

278.27

54.25

5.13

unknown protein

blr3795

TGCATTGCAGCG

40.09

19.03

2.11

ABC transporter HlyB/MsbA family

blr3904

TGCAGTGCTGCA

312.33

109.92

2.84

probable iron transport protein

blr4188

CGCAGTGCAGCA

28.85

12.98

2.22

hypothetical protein

bll4430

TGCAGCGCAGCA

104.34

38.21

2.73

hypothetical protein

bsr4431

TGCAGCGCAGCA

216.36

77.85

2.78

hypothetical protein

pdhA (bll4783)

TGCAGTGCGGCA

249.67

111.10

2.25

pyruvate dehydrogenase alpha subunit

bll4833

TGCGGCGCACCA

530.73

140.09

3.79

unknown protein

bsr4834

TGCGGCGCACCA

168.95

52.96

3.19

unknown protein

blr4841

TGCCGCGCACAA

30.64

14.59

2.10

unknown protein

bll4952

TGCATCGCACAA

27.07

7.50

3.61

NfeD protein homolog

bll5160

TGCGGCGCACAA

106.54

46.40

2.30

conserved hypothetical protein; putative alpha/beta-Hydrolases

bsl5321

CGCGGCGCAGCA

415.34

205.51

2.02

unknown protein

bll5335

TGCGCCGGACAA

35.29

16.85

2.09

putative thiolase

blr5540

TGCGGTGCCCAA

231.26

58.07

3.98

hypothetical protein

bsl5717

TGCGGCGCCCAA

109.78

34.94

3.14

hypothetical protein

bll5961

TGCGGTGCAACA

12.66

4.23

2.99

transcriptional regulatory protein Crp family

blr5962

TGCGGTGCAACA

40.98

7.40

5.54

ABC transporter ATP-binding protein

bll6121

TGCAGCGCACAA

21.88

9.39

2.33

probable sulfite oxidase

bll6206

CGCGCCGCACAA

74.19

35.28

2.10

hypothetical protein

exaA (blr6207)

CGCGCCGCACAA

93.32

23.85

3.91

probable quinoprotein ethanol dehydrogenase precursor

blr6443

TGCAATGCAACA

13.79

2.83

4.87

ABC transporter permease protein

blr6465

CGCGATGCACAA

39.03

14.29

2.73

putative steroid monooxygenase

bll6733

TGCGACGAAGCA

45.09

21.88

2.06

putative amidase

pqqA (bsr6735)

TGCAGTGCAACA

156.84

40.03

3.92

putative pyrroloquinoline quinone synthesis protein A

blr6837

CGCAGTGCAGCA

34.10

7.30

4.67

hypothetical protein

blr6886

CGCATTGCACAA

57.55

25.47

2.26

transcriptional regulatory protein MarR family

bll7487

TGCGGAGCACAA

456.33

176.20

2.59

unknown protein

bll7511

TGCAACGCAGAT

167.13

62.44

2.68

unknown protein

bll7663

TGCACCGCAGCA

57.90

24.14

2.40

unknown protein

pckA (bll8141)

TGCGACGCACAA

386.03

107.43

3.59

phosphoenolpyruvate carboxykinase

  1. aFold change: USDA110 RPKM/ΔphaR RPKM