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Table 1 Bacterial strains and plasmids used in this study

From: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation

Strains or plasmids

Derivation and relevant properties

Source of reference

B. diazoefficiens

 USDA110

Wild type

[42]

 ΔphaR

A mutant of USDA110 with deletion of phaR

This work

E. coli

 DH5α

supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Takara Bio

 S17–1

F- thi pro hsdR [RP4–2 tet::Mu kan::Tn7 (trp str)]

[43]

 BL21(DE3)

F− ompT hadSβ (rβ− mβ-) gal dcm (DE3)

Takara Bio

Plasmids

 pK18mobsacB

Mobilizable plasmid containing oriV, oriT, mob, sacB, and kan

[44]

 pK18mobsacBΔphaR

pK18 mobsacB containing the flanking regions of phaR

This work

 pET28b

kan

Takara Bio

 pET28PhaR

pET28b carrying the coding region of phaR

This work

 pMD20

A linearized vector for TA cloning of PCR fragments

Takara Bio