Strains or plasmids | Derivation and relevant properties | Source of reference |
---|---|---|
B. diazoefficiens | ||
 USDA110 | Wild type | [42] |
 ΔphaR | A mutant of USDA110 with deletion of phaR | This work |
E. coli | ||
 DH5α | supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Takara Bio |
 S17–1 | F- thi pro hsdR [RP4–2 tet::Mu kan::Tn7 (trp str)] | [43] |
 BL21(DE3) | F− ompT hadSβ (rβ− mβ-) gal dcm (DE3) | Takara Bio |
Plasmids | ||
 pK18mobsacB | Mobilizable plasmid containing oriV, oriT, mob, sacB, and kan | [44] |
 pK18mobsacBΔphaR | pK18 mobsacB containing the flanking regions of phaR | This work |
 pET28b | kan | Takara Bio |
 pET28PhaR | pET28b carrying the coding region of phaR | This work |
 pMD20 | A linearized vector for TA cloning of PCR fragments | Takara Bio |