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Table 1 Bacterial strains and plasmids used in this study

From: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation

Strains or plasmids Derivation and relevant properties Source of reference
B. diazoefficiens
 USDA110 Wild type [42]
 ΔphaR A mutant of USDA110 with deletion of phaR This work
E. coli
 DH5α supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Takara Bio
 S17–1 F- thi pro hsdR [RP4–2 tet::Mu kan::Tn7 (trp str)] [43]
 BL21(DE3) F ompT hadSβ (rβ-) gal dcm (DE3) Takara Bio
Plasmids
 pK18mobsacB Mobilizable plasmid containing oriV, oriT, mob, sacB, and kan [44]
 pK18mobsacBΔphaR pK18 mobsacB containing the flanking regions of phaR This work
 pET28b kan Takara Bio
 pET28PhaR pET28b carrying the coding region of phaR This work
 pMD20 A linearized vector for TA cloning of PCR fragments Takara Bio