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Table 2 C3b/iC3b assay variability for strains 3655, 3224A and MPJ003 (14 sera per strain in triplicate, n = 126)

From: Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzae

  Description Sera with defined CV Mean CV (±SEM)
< 20% < 35% < 40% > 40%
Intra-assay variability
n = 126
3 plates,
1 operator,
1 day
34 4 1 3 13.09 (2.27)
Inter-assay variability
n = 126
1 plate,
1 operator,
3 days
33 7 2 0 11.40 (1.55)
Inter-operator variability
n = 126
1 plate,
3 operators,
1 day
26 6 3 7 21.47 (2.67)