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Table 2 C3b/iC3b assay variability for strains 3655, 3224A and MPJ003 (14 sera per strain in triplicate, n = 126)

From: Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzae

 

Description

Sera with defined CV

Mean CV (±SEM)

< 20%

< 35%

< 40%

> 40%

Intra-assay variability

n = 126

3 plates,

1 operator,

1 day

34

4

1

3

13.09 (2.27)

Inter-assay variability

n = 126

1 plate,

1 operator,

3 days

33

7

2

0

11.40 (1.55)

Inter-operator variability

n = 126

1 plate,

3 operators,

1 day

26

6

3

7

21.47 (2.67)