Fig. 4From: Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzaeSelection of complement concentration for CDA. NTHi strain 3655 was incubated for 45Â min with 2% or 25% IgG-depleted plasma in the presence of human pre and post human Synflorix sera. Antibody-dependent (a) C3b/iC3b and (b) C5b-9 binding was determined by flow cytometry. Significance values were determined by two tailed t-testBack to article page