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Fig. 1 | BMC Microbiology

Fig. 1

From: Novel human monoclonal antibodies targeting the F subunit of leukocidins reduce disease progression and mortality caused by Staphylococcus aureus

Fig. 1

Rabbit RBCs and human leukocytes were susceptible to γ-hemolysin. (a) HlgAB and HlgBC induced the hemolysis of rabbit red blood cells (rRBCs) in a concentration-dependent manner. (b–d) HlgAB induced the hemolysis of mouse red blood cells (mRBCs) (b), sheep red blood cells (sRBCs) (c), and human red blood cells (hRBCs) (d) in a concentration-dependent manner. Serial dilutions of HlgAB and HlgBC proteins were incubated with 2% (v/v) solution of erythrocytes at 37 °C for 1 h. The samples were centrifuged, and the absorbance of the supernatants was measured at 405 nm. (e) HlgAB and HlgCB efficiently induced cell lysis of human neutrophils and monocytes. Leukocytes were isolated from a healthy donor, incubated with PBS or γ-hemolysin at room temperature for 1 h, and gated for CD14 and CD11b positivity. The percentages of human neutrophils and monocytes were determined by FCM

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BMC Microbiology

ISSN: 1471-2180

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