Fig. 5
From: Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K
Molecular mass determination of VDH from Streptomyces sp. NL15-2K. a SDS-PAGE analysis of proteins from each purification step. Lane M: molecular mass markers (sizes indicated); lane 1: cell-free extract from E. coli harboring pET28a/VDH; lane 2: active fraction from HiPrep DEAE FF; lane 3: active fraction from RESOURCE PHE; lane 4: active fraction from Mono Q. b Native molecular mass determination of VDH through gel-filtration analysis. Open circle: VDH; closed circles: molecular mass standards